EMPIAR-11797 - Single-Particle Cryo-EM of RNA Polymerase at Various Tilts [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11797

Single-Particle Cryo-EM of RNA Polymerase at Various Tilts [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Overcoming Resolution Attenuation During Tilted Cryo-EM Data Collection

Aiyer S, Baldwin PR, Tan SM, Shan Z, Oh J, Mehrani A, Bowman ME, Louie G, Passos DO, Đorđević-Marquardt S, Mietzsch M, Hull JA, Hoshika S, Barad BA, Grotjahn DA, McKenna R, Agbandje-McKenna M, Benner SA, Noel JAP, Wang D, Tan YZ, Lyumkis D

bioRxiv (---)

DOI: 10.1101/2023.07.14.548955

Related EMDB entry:

EMD-41695

Deposited:

2023-11-27

Released:

2024-03-26

Last modified:

2024-03-26

Imageset size:

487.35 GB

Imageset DOI:

10.6019/EMPIAR-11797

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. Unaligned multiframe movies of RNA polymerase collected at various tilts

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1146

Image size:

(4092, 5760)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 50 frames were used, pixel size is 0.830 Å/pixel, total dose of 34.2 e-/Å2 (0 degrees) and 38.8 e-/Å2 (60 degrees). Gain reference is 22nov04c_04153642_01_4092x5760_norm_0.mrc.

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2. Aligned and dose-weighted micrographs of RNA polymerase collected at various tilts

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

1146

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.83, 0.83)

Details:

Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.

Files:

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3. Polished and equalized particles of RNA polymerase collected at various tilts

Category:

picked particles - multiframe - processed

Image format:

MRCS

No. of images or tilt series:

Image size:

(312, 312)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.83, 0.83)

Details:

Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.

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