Movies collected using Titan Krios and K3 quantum with energy filter. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders. 50 frames were used, pixel size is 0.830 Å/pixel, total dose of 34.2 e-/Å2 (0 degrees) and 38.8 e-/Å2 (60 degrees). Gain reference is 22nov04c_04153642_01_4092x5760_norm_0.mrc.
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Aligned and dose weighted using UCSF MotionCor2. Tilt angles of 0, 10, 20, 30, 40, 50 and 60 degrees were collected put into their respective folders.
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Final particle stack after particle polishing using Relion. The particles are equalized so that there are same number of particles per tilt angle with similar defocus distribution.
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