EMPIAR-11522
CryoEM of E. coli RNA polymerase Sigma N bound to full duplex DNA fragment dhsU36 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

A general mechanism for transcription bubble nucleation in bacteria

Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 36972428

Related EMDB entry:
Deposited:
2023-04-06
Released:
2023-05-16
Last modified:
2023-05-16
Imageset size:
2.43 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned micrographs (raw movies)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
6088
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.083, 1.083)
Details:
FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit; accelerating voltage 300 kV; dose 25.7 e-/px; pixel size 1.083 A/px; exposure 2.0 s; dose/frame 0.87647 e-/A^2/frame; frame time 0.05 s; total dose 43.82 e-/A^2; total frames 40; defocus range -0.8 to -2.5um
Gain references can be found in the "references" subfolder.
Picked particles star file "EsN-dhsU36full_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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2. Motion-corrected micrographs (dose-weighted)
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
6088
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
See also the "corrected_micrographs.star" file in the "aux_files" folder.
Picked particles star file "EsN-dhsU36full_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.
Files:
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3. Particle images for map 1
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
5836
Image size:
(324, 324)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36full_particles_map1.star" (final refinement parameters) and "EsN-dhsU36full_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)
Files:
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4. Particle images for map 2 (subtracted particles)
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
558
Image size:
(324, 324)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.083, 1.083)
Details:
Particle stack for map 2 after masked subtraction (see "EsN-dhsU36full_particles_map2.star" (final refinement parameters) and "EsN-dhsU36full_particles_original_map2.star" (original particles) in "aux_files" folder)
Files:
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Files:
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Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)