EMPIAR-11522 - CryoEM of E. coli RNA polymerase Sigma N bound to full duplex DNA fragment dhsU36 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11522

CryoEM of E. coli RNA polymerase Sigma N bound to full duplex DNA fragment dhsU36 (-12T) [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

A general mechanism for transcription bubble nucleation in bacteria

Mueller AU, Chen J, Wu M, Chiu C, Nixon BT, Campbell EA, Darst SA

Proceedings of the National Academy of Sciences of the United States of America 120 (2023)

PMID: 36972428

DOI: 10.1073/pnas.2220874120

Related EMDB entry:

EMD-28786 EMD-28791 EMD-28790

Deposited:

2023-04-06

Released:

2023-05-16

Last modified:

2023-05-16

Imageset size:

2.43 TB

Imageset DOI:

10.6019/EMPIAR-11522

Experimental metadata:

Download xml

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned micrographs (raw movies)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6088

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.083, 1.083)

Details:

FEI Titan Krios + Gatan K3, super-resolution, image shift, BioQuantum energy filter 20 eV slit; accelerating voltage 300 kV; dose 25.7 e-/px; pixel size 1.083 A/px; exposure 2.0 s; dose/frame 0.87647 e-/A^2/frame; frame time 0.05 s; total dose 43.82 e-/A^2; total frames 40; defocus range -0.8 to -2.5um
Gain references can be found in the "references" subfolder.
Picked particles star file "EsN-dhsU36full_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.

Files:

2. Motion-corrected micrographs (dose-weighted)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6088

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

See also the "corrected_micrographs.star" file in the "aux_files" folder.
Picked particles star file "EsN-dhsU36full_particles_picked.star" ("aux_files" folder) contains all particles picked initially and is only provided for reference.

Files:

3. Particle images for map 1

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

5836

Image size:

(324, 324)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

Particle stack for map 1 after Bayesian polishing (see "EsN-dhsU36full_particles_map1.star" (final refinement parameters) and "EsN-dhsU36full_particles_shiny.star" (output from Bayesian Polishing) in "aux_files" folder)

Files:

4. Particle images for map 2 (subtracted particles)

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

558

Image size:

(324, 324)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.083, 1.083)

Details:

Particle stack for map 2 after masked subtraction (see "EsN-dhsU36full_particles_map2.star" (final refinement parameters) and "EsN-dhsU36full_particles_original_map2.star" (original particles) in "aux_files" folder)

Files: