EMPIAR-11364 - Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer [1964 multi-frame micrographs composed of 30 frames each in MRC format]

EMPIAR-11364

Cryogenic electron tomographs of Coxsackievirus A9 treated with endosomal ionic condition buffer [1964 multi-frame micrographs composed of 30 frames each in MRC format]

Publication:

Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release

Domanska A, Plavec Z, Ruokolainen V, Löflund B, Marjomäki V, Butcher SJ

Journal of virology 96 (2022)

PMID: 36448797

DOI: 10.1128/jvi.01367-22

Related EMDB entry:

EMD-15706

Deposited:

2022-12-12

Released:

2023-01-20

Last modified:

2023-01-20

Imageset size:

1.80 TB

Imageset DOI:

10.6019/EMPIAR-11364

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Raw micrographs of CVA9 treated with ionic buffer condtions 3h

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1964

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.24, 1.24)

Details:

Micrographs were collected on FEI TAlos Arctica operating at 200 kV with a Falcon III direct electron detector at a nominal magnification of ×120,000. Each movie consisted of 30 frames with an exposure time of 1 s per frame. The total electron dose was approximately 30 electrons per Å2.

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