EMPIAR-10862 - Cryo-EM of recombinant 201phi2-1 chimallin [4058 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10862

Cryo-EM of recombinant 201phi2-1 chimallin [4058 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25391 EMD-25392 EMD-25390 EMD-25393

Deposited:

2021-11-07

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

679.86 GB

Imageset DOI:

10.6019/EMPIAR-10862

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned frames as unormalized LZW-TIFF

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4058

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.075, 1.075)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 degree CW rotation prior to application to TIFs. Each frame-series is 40 frames with cumulative fluence of 42.6 electrons per square Angstrom. Particle metadata for the localized tetramer stage in RELION-v3.1 format and for the fully expanded protomer stage in cryoSPARC-v3.1 format in data/particle_metadata.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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