EMPIAR-10862 - Cryo-EM of recombinant 201phi2-1 chimallin [4058 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10862

Cryo-EM of recombinant 201phi2-1 chimallin [4058 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25391 EMD-25392 EMD-25390 EMD-25393

Deposited:

2021-11-07

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

679.86 GB

Imageset DOI:

10.6019/EMPIAR-10862

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned frames as unormalized LZW-TIFF

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4058

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.075, 1.075)

Details:

Gain reference supplied in data/frames as MRC and requires a 270 degree CW rotation prior to application to TIFs. Each frame-series is 40 frames with cumulative fluence of 42.6 electrons per square Angstrom. Particle metadata for the localized tetramer stage in RELION-v3.1 format and for the fully expanded protomer stage in cryoSPARC-v3.1 format in data/particle_metadata.

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

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