EMPIAR-10711
Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:
Deposited:
2021-05-11
Released:
2022-06-14
Last modified:
2022-06-14
Imageset size:
2.11 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4591
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.536, 0.536)
Details:
Contents are as follows:
raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
201216_struct_A_GainRef.mrc == gain reference for the dataset
micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr_metadata/ == metadata of the motion correction step
==
cryolo_PhosaurusNet_RNF213_config.h5 == cryolo network model used for initial particles picking
cryolo_PhosaurusNet_RNF213_config.json == config file for the cryolo network model used for initial particles picking
coordinates_initial_cryolo/ == particle coordinates picked by cryolo
coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
refined_particles.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset
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