EMPIAR-10711 - Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10711

Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:

EMD-12931

Deposited:

2021-05-11

Released:

2022-06-14

Last modified:

2022-06-14

Imageset size:

2.11 TB

Imageset DOI:

10.6019/EMPIAR-10711

Experimental metadata:

Download xml

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4591

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.536, 0.536)

Details:

Contents are as follows:
raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
201216_struct_A_GainRef.mrc == gain reference for the dataset
micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr_metadata/ == metadata of the motion correction step
==
cryolo_PhosaurusNet_RNF213_config.h5 == cryolo network model used for initial particles picking
cryolo_PhosaurusNet_RNF213_config.json == config file for the cryolo network model used for initial particles picking
coordinates_initial_cryolo/ == particle coordinates picked by cryolo
coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
refined_particles.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset

Files: