EMPIAR-10711
Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:
Deposited:
2021-05-11
Released:
2022-06-14
Last modified:
2022-06-14
Imageset size:
2.11 TB
Imageset DOI:
Experimental metadata:
Contains:
  • micrographs - multiframe
1. unaligned multi-frame micrographs
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
4591
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.536, 0.536)
Details:
Contents are as follows:
raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
201216_struct_A_GainRef.mrc == gain reference for the dataset
micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr_metadata/ == metadata of the motion correction step
==
cryolo_PhosaurusNet_RNF213_config.h5 == cryolo network model used for initial particles picking
cryolo_PhosaurusNet_RNF213_config.json == config file for the cryolo network model used for initial particles picking
coordinates_initial_cryolo/ == particle coordinates picked by cryolo
coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
refined_particles.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset
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Citations
Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)
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