EMPIAR-10711 - Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]

This entry has been removed from PDBj storage. Please download it from EMPIAR-EBI.

EMPIAR-10711

Transmission electron micrographs of mouse RNF213 in a chemically-stabilized complex with human UBE2L3 [4591 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP

Ahel J, Clausen T, Virdee S, Fletcher AJ, Grabarczyk DB, Deszcz L, Lehner A, Roitinger E

BiorXiv (---)

Related EMDB entry:

EMD-12931

Deposited:

2021-05-11

Released:

2022-06-14

Last modified:

2022-06-14

Imageset size:

2.11 TB

Imageset DOI:

10.6019/EMPIAR-10711

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4591

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.536, 0.536)

Details:

Contents are as follows:
raw_micrographs/ == unaligned multi-frame micrographs, not gain-corrected
==
201216_struct_A_GainRef.mrc == gain reference for the dataset
micrographs_ctf.star == relion 3.1 star file describing the gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr/ == gain-corrected dose-weighed motion-corrected micrographs
micrographs_ctf_motioncorr_metadata/ == metadata of the motion correction step
==
cryolo_PhosaurusNet_RNF213_config.h5 == cryolo network model used for initial particles picking
cryolo_PhosaurusNet_RNF213_config.json == config file for the cryolo network model used for initial particles picking
coordinates_initial_cryolo/ == particle coordinates picked by cryolo
coordinates_final_refined/ == particle coordinates for the particles used in the final reconstruction
particles/ == particles used in the final reconstruction, Bayesian-polished by relion 3.1
refined_particles.star == relion 3.1 star file describing the particles after the final reconstruction, including angular assignment
==
mtf_k3_standard_300kV_FL2.star == MTF file used for MTF correction for this dataset

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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