EMPIAR-10252 - Catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [4812 multi-frame micrographs composed of 44 frames each in MRC format]

EMPIAR-10252

Catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 movies obtained using Talos Arctica operating at 200 kV equipped with a K2 [4812 multi-frame micrographs composed of 44 frames each in MRC format]

Publication:

High-resolution structure determination of sub-100 kilodalton complexes using conventional cryo-EM

Herzik Jr MA, Wu M, Lander GC

bioRxiv (2018)

DOI: 10.1101/489898

Related EMDB entry:

EMD-0409

Deposited:

2019-02-11

Released:

2019-03-25

Last modified:

2019-04-12

Imageset size:

619.55 GB

Imageset DOI:

10.6019/EMPIAR-10252

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned movie stacks of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 acquired on a Talos Arctica using a K2 direct electron detector

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4812

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.5585, 0.5585)

Details:

Raw, unaligned counting mode frame stacks (44 frames) of the catalytic subunit of protein kinase A bound to ATP, manganese, and IP20 in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector. Three separate data collections – 18feb27c (collected without tilt), 18mar03b (collected without tilt), and 18aug21a (collected at a 30˚ tilt) – that were combined are provided. Images used for corrections are also provided (require flip in Y).

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