EMPIAR-13510
Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides [multiple data sets in MRC and MRCS formats]
Publication:

Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides

Ghosh Dastidar N, Freyer NS, Petrychenko V, de A P Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi AZ, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I, Ghosh Dastidar N, Freyer NS, Petrychenko V, de A P Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi AZ, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I

Nature communications 16 (2025)

PMID: 41162373

Related EMDB entry:
Deposited:
2025-10-27
Released:
2026-05-31
Last modified:
2026-05-31
Imageset size:
1.52 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - single frame - processed
1. Motion-corrected, dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
16025
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Note: Micrographs were obtained from movie-stacks of 20 frames à 1.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "corrected_micrographs.star"
Files:
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2. Shiny particles of non-rotated ribosome particles, obtained by Bayesian polishing in Relion
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
1417920
Image size:
(288, 288)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Star-files of shiny particles:
- C (EMD-55123): "particles_C.star"
Files:
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3. Shiny particles of rotated ribosome particles, obtained by Bayesian polishing in Relion
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
303206
Image size:
(288, 288)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Star-files of shiny particles:
- H1 (EMD-55124): "particles_H1.star"
- H1-EF-G (EMD-54253): "particles_H1-EF-G.star"
- H2 (EMD-55125): "particles_H2.star"
- H2-EF-G (EMD-54254): "particles_H2-EF-G.star"
Files:
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