EMPIAR-13510 - Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides [multiple data sets in MRC and MRCS formats]

EMPIAR-13510

Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides [multiple data sets in MRC and MRCS formats]

Publication:

Selective silencing of antibiotic-tethered ribosomes as a resistance mechanism against aminoglycosides

Ghosh Dastidar N, Freyer NS, Petrychenko V, de A P Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi AZ, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I, Ghosh Dastidar N, Freyer NS, Petrychenko V, de A P Schwarzer AC, Peng BZ, Samatova E, Kothe C, Schmidt M, Peske F, Politi AZ, Urlaub H, Fischer N, Rodnina MV, Wohlgemuth I

Nature communications 16 (2025)

PMID: 41162373

DOI: 10.1038/s41467-025-65298-7

Related EMDB entry:

EMD-54254 EMD-55124 EMD-55123 EMD-54253

Deposited:

2025-10-27

Released:

2026-05-31

Last modified:

2026-05-31

Imageset size:

1.52 TB

Imageset DOI:

10.6019/EMPIAR-13510

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Motion-corrected, dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

16025

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Note: Micrographs were obtained from movie-stacks of 20 frames à 1.5 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "corrected_micrographs.star"

Files:

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2. Shiny particles of non-rotated ribosome particles, obtained by Bayesian polishing in Relion

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

1417920

Image size:

(288, 288)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Star-files of shiny particles:
- C (EMD-55123): "particles_C.star"

Files:

This entry cannot be downloaded from PDBj.

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3. Shiny particles of rotated ribosome particles, obtained by Bayesian polishing in Relion

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

303206

Image size:

(288, 288)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Star-files of shiny particles:
- H1 (EMD-55124): "particles_H1.star"
- H1-EF-G (EMD-54253): "particles_H1-EF-G.star"
- H2 (EMD-55125): "particles_H2.star"
- H2-EF-G (EMD-54254): "particles_H2-EF-G.star"

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)