EMPIAR-13386
Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed [multiple data sets in MRC and MRCS formats]
Publication:

Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed

Schöndorf T, Petrychenko V, Kotan I, Dahal D, Napieraj N, Cruz-Zaragoza LD, Wang C, Urbach O, Gall T, Dennerlein S, Kramer G, Fischer N, Rehling P

Nature structural & molecular biology 33 (2026) 853-867

PMID: 42098403

Related EMDB entry:
Deposited:
2026-03-18
Released:
2026-05-29
Last modified:
2026-05-29
Imageset size:
3.47 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - single frame - processed
1. Motion-corrected, dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
51805
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Note: Micrographs were obtained from movie-stacks of 30-40 frames à ~1 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "micrographs.star"
Files:
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2. Shiny ribosome particles, obtained by Bayesian polishing in Relion
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
533455
Image size:
(400, 400)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.16, 1.16)
Details:
Star-files of shiny particles:
- P (EMD-55836): "particles_P.star"
- AP (EMD-55837): "particles_AP.star"
- AP* (EMD-54637): "particles_APstar.star"
- H1 (EMD-54638): "particles_H1.star"
- H2 (EMD-55838): "particles_H2.star"
- open COX1-mtRNC-OXA1L/MITRAC (EMD-54639): "particles_COX1-mtRNC-OXA1LMITRAC_open.star"
- closed COX1-mtRNC-OXA1L/MITRAC (EMD-54640): "particles_COX1-mtRNC-OXA1LMITRAC_closed.star"
Masks used for the RELION focused classifications are located in data/data/Masks
Files:
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