EMPIAR-13386 - Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed [multiple data sets in MRC and MRCS formats]

EMPIAR-13386

Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed [multiple data sets in MRC and MRCS formats]

Publication:

Membrane insertion of mitochondrial-encoded proteins regulates ribosome decoding speed

Schöndorf T, Petrychenko V, Kotan I, Dahal D, Napieraj N, Cruz-Zaragoza LD, Wang C, Urbach O, Gall T, Dennerlein S, Kramer G, Fischer N, Rehling P

Nature structural & molecular biology 33 (2026) 853-867

PMID: 42098403

DOI: 10.1038/s41594-026-01803-w

Related EMDB entry:

Deposited:

2026-03-18

Released:

2026-05-29

Last modified:

2026-05-29

Imageset size:

3.47 TB

Imageset DOI:

10.6019/EMPIAR-13386

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Motion-corrected, dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

51805

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Note: Micrographs were obtained from movie-stacks of 30-40 frames à ~1 e/A^2 by global motion-correction and dose-weighting using MotionCor2 in Relion.
- Micrographs .star-file: "micrographs.star"

Files:

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2. Shiny ribosome particles, obtained by Bayesian polishing in Relion

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

533455

Image size:

(400, 400)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.16, 1.16)

Details:

Star-files of shiny particles:
- P (EMD-55836): "particles_P.star"
- AP (EMD-55837): "particles_AP.star"
- AP* (EMD-54637): "particles_APstar.star"
- H1 (EMD-54638): "particles_H1.star"
- H2 (EMD-55838): "particles_H2.star"
- open COX1-mtRNC-OXA1L/MITRAC (EMD-54639): "particles_COX1-mtRNC-OXA1LMITRAC_open.star"
- closed COX1-mtRNC-OXA1L/MITRAC (EMD-54640): "particles_COX1-mtRNC-OXA1LMITRAC_closed.star"
Masks used for the RELION focused classifications are located in data/data/Masks

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)