EMPIAR-13378 - Clostridium butyricum Argonaute wild type apo protein complexed with de novo designed binder2 [7097 multi-frame micrographs composed of 50 frames each in EER format]

EMPIAR-13378

Clostridium butyricum Argonaute wild type apo protein complexed with de novo designed binder2 [7097 multi-frame micrographs composed of 50 frames each in EER format]

Publication:

Target-DNA binding-induced dimerization is required for catalytic activation of Clostridium butyricum Argonaute

Barendse P, van Drimmelen M, Pacesa M, Sivaraman A, Zhang DK, Nickel L, Niault T, Lindhoud S, Roosjen M, Hegge JW, Park K, Correia BE, van der Oost J, Joo C, Westphal AH, Swarts DC

(2026)

Related EMDB entry:

Deposited:

2026-03-14

Released:

2026-03-19

Last modified:

2026-03-19

Imageset size:

3.06 TB

Imageset DOI:

10.6019/EMPIAR-13378

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Movies of CbAgo wild type in apo form, complexed with de novo designed binder2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7097

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.658, 0.658)

Details:

Sample of wild type Clostridium butyricum Argonaute apo protein mixed with BindCraft designed de novo binder2 (SEDYEMIELFSAQFIWLIAHDIAKMEGWENEYLHEVIDYMDKIIEKIEEKYGNQDPNELFEKHFNSWSKEKQEKFRKVFDELFDKLMKEIGFFDWPNLDAKEFAERLKKAHEKLVEELAKALGIKP).
Purified in buffer 20 mM Tris-HCl pH 7.5, 250 mM KCl, 5% glycerol.
50 frame movies, with a 50 e/A^2 electron dose, on a 300 kV Titan Krios with Falcon 4i detector, with 2.7 mm C2 lens, collected with a 0.658 A pixel size.

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