EMPIAR-13270
Cryo electron tomography data presented in Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites [multiple data sets in MRC format]
Publication:

Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites

Downes K, Flood J, Nans A, Van der Verren S, Audhya A, Zanetti G

bioRxiv (2025)

Related EMDB entry:
Deposited:
2025-10-21
Released:
2026-04-20
Last modified:
2026-04-20
Imageset size:
637.74 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • reconstructed volumes
  • micrographs - multiframe
  • tilt series
1. Denoised tomograms containing COPII
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
63
Image size:
(896, 896)
Pixel type:
16 BIT FLOAT
Pixel spacing:
(10.0, 10.0)
Details:
The 63 tilt series containing regions of interest were processed following the WarpTools pipeline, including motion correction, CTF and defocus handedness estimation (https://warpem.github.io/warp/). Tilt series were aligned using the WarpTools etomo patch tracking wrapper. Bad tilts were manually removed, and poorly aligned tilt series were manually improved with etomo. CTF-corrected tomograms were reconstructed at a pixel size of 10 Å and used to pick ribosomes for further improvement of alignments with Warp/M. Cryocare was used to denoise the tomograms.
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2. Raw frames for the COPII containing tomograms
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
63
Image size:
(3710, 3838)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(2.24, 2.24)
Details:
Raw frames of the 63 COPII containing tilt series. Each tilt series contains 42 tilts. Also included is the star file for the ribosome picking which yielded a 15A ribosome map that was further used to improve tilt series alignments for tomogram reconstruction via the Warp/M pipeline.
Files:
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3. mdocs
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
63
Image size:
(3710, 3838)
Pixel type:
BIT
Pixel spacing:
(2.24, 2.24)
Details:
mdocs for the 63 COPII containing tomograms.
Files:
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