Cryo electron tomography data presented in Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites [multiple data sets in MRC format]
Publication:
Multi-scale Molecular Imaging of Human Cells reveals COPI and COPII Vesicles at ER Exit Sites
The 63 tilt series containing regions of interest were processed following the WarpTools pipeline, including motion correction, CTF and defocus handedness estimation (https://warpem.github.io/warp/). Tilt series were aligned using the WarpTools etomo patch tracking wrapper. Bad tilts were manually removed, and poorly aligned tilt series were manually improved with etomo. CTF-corrected tomograms were reconstructed at a pixel size of 10 Å and used to pick ribosomes for further improvement of alignments with Warp/M. Cryocare was used to denoise the tomograms.
Files:
Available download options:
This entry cannot be downloaded from PDBj.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Raw frames of the 63 COPII containing tilt series. Each tilt series contains 42 tilts. Also included is the star file for the ribosome picking which yielded a 15A ribosome map that was further used to improve tilt series alignments for tomogram reconstruction via the Warp/M pipeline.
Files:
Available download options:
This entry cannot be downloaded from PDBj.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
