Raw unaligned frames were preprocessed in Warp to generate tilt series stacks. Corresponding reference files in other directories under same base name (tilt series, mdocs, tomograms). Four directories included in subfolders. MIM019_2 and MIM019_3 are from mtGFP mouse embryonic fibroblasts (untreated). MIM029_3 and MIM032_1 are from mtGFP mouse embryonic fibroblasts treated with degraded Thapsigargin (500nm) for 8 hours- condition uncertain.
The tilt series stacks (.st file) and rawtlt files (.rawtlt file) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the Tilt_series_mdoc folder. Tilt series stacks are then aligned in IMOD. MIM019_2 and MIM019_3 are from mtGFP mouse embryonic fibroblasts (untreated). MIM029_3 and MIM032_1 are from mtGFP mouse embryonic fibroblasts treated with degraded Thapsigargin (500nm) for 8 hours- condition uncertain.
Bin 8 Warp reconstructed tomograms. Subset of MIM19_2, MIM019_3 used to generated organelle membrane segmentation. Associated segmentations files can be found in data/morphometrics_thickness/Voxel_segmentations. Associated triangulated surface meshes can be found in /data/morphometrics_thickness/Triangulated_surface_mesh.
The tilt series stacks (.st file) and rawtlt files (in Tilt_series_rawtlt) were created from Warp with the input of tilt-corrected mdoc files. Raw mdoc files are listed in the yeast/tilt_series_mdocs folder. Tilt series stacks are then aligned in IMOD.
voxel segmentations from reconstructed tomograms at bin 8. These voxel segmentations were then input into surface morphometrics to generate triangulated surface meshes.
