EMPIAR-12917 - Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment [2511 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-12917

Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment [2511 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

Henriques WS, Bowman J, Hall LN, Gauvin CC, Wei H, Kuang H, Zimanyi CM, Eng ET, Santiago-Frangos A, Wiedenheft B

bioRxiv (2025)

PMID: 40661357

DOI: 10.1101/2025.06.10.658980

Related EMDB entry:

EMD-71097

Deposited:

2025-08-10

Released:

2025-11-04

Last modified:

2025-11-04

Imageset size:

1.74 TB

Imageset DOI:

10.6019/EMPIAR-12917

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

--- file not found ---

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a fragment of dsDNA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2511

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8365, 0.8365)

Details:

40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.

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Citations

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Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

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Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)