EMPIAR-12917
Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment [2511 multi-frame micrographs composed of 40 frames each in MRC format]
Publication:

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

Henriques WS, Bowman J, Hall LN, Gauvin CC, Wei H, Kuang H, Zimanyi CM, Eng ET, Santiago-Frangos A, Wiedenheft B

bioRxiv (2025)

PMID: 40661357

Related EMDB entry:
Deposited:
2025-08-10
Released:
2025-11-04
Last modified:
2025-11-04
Imageset size:
1.74 TB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (rawdata/*.frames.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   3,291,308,320 kilobytes
Compressed size: 1,868,904,961 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a fragment of dsDNA
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2511
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(0.8365, 0.8365)
Details:
40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.
Files:
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He L, Bartesaghi A. (2026)
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
Jones HN, Deshmukh A, Pande K. (2026)
Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)
Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)
Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)
Geißler K, Kreysing JP, Wang Y, Glushkova D, Obarska-Kosinska A, Hoffmann PC, Böhm S, Schmidt A, Meier-Credo J, Langer JD, Hummer G, Beck M. (2026)