EMPIAR-12917 - Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment [2511 multi-frame micrographs composed of 40 frames each in MRC format]

EMPIAR-12917

Cas1-Cas2/3 heterohexamer bound to foreign dsDNA fragment [2511 multi-frame micrographs composed of 40 frames each in MRC format]

Publication:

Structures reveal how the Cas1-2/3 integrase captures, delivers, and integrates foreign DNA into CRISPR loci

Henriques WS, Bowman J, Hall LN, Gauvin CC, Wei H, Kuang H, Zimanyi CM, Eng ET, Santiago-Frangos A, Wiedenheft B

bioRxiv (2025)

PMID: 40661357

DOI: 10.1101/2025.06.10.658980

Related EMDB entry:

EMD-71097

Deposited:

2025-08-10

Released:

2025-11-04

Last modified:

2025-11-04

Imageset size:

1.74 TB

Imageset DOI:

10.6019/EMPIAR-12917

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

--- file not found ---

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of the Cas1-2/3 heterohexameric complex bound to a fragment of dsDNA

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2511

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(0.8365, 0.8365)

Details:

40 frame movies were collected at a 150 ms frame rate and a dose rate of 8.63 e-/Å2/s for a total dose of 51.78 e-/Å2 with a pixel size of 0.833 Å/px over a defocus range of -0.9 to -2.8 um.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)