EMPIAR-12582 - Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes [854 multi-frame micrographs composed of 33 frames each in MRC format]

EMPIAR-12582

Pichia pastoris mitochondrial complex I reconstituted into proteoliposomes [854 multi-frame micrographs composed of 33 frames each in MRC format]

Publication:

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J

Science advances 11 (2025)

PMID: 41032597

DOI: 10.1126/sciadv.adz0693

Related EMDB entry:

EMD-52893 EMD-52892 EMD-52891 EMD-52890

Deposited:

2025-02-24

Released:

2025-10-30

Last modified:

2025-10-30

Imageset size:

537.11 GB

Imageset DOI:

10.6019/EMPIAR-12582

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to proteoliposomes.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

854

Image size:

(4096, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.37, 1.37)

Details:

Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to proteoliposomes. Frozen on Qunatifoil gold grids. Gain-corrected mages (MRC) were collected with a 200 keV FEI Talos Arctica on a Falcon 3EC detector. The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 1.69 s exposures were captured in 33 frames with a total dose of 50.37 e-/Å-2. A pixel size of 1.37 Å/pixel was used.

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