Unaligned multi-frame micrographs of Pichia pastoris (Komagataella phaffii) mitochondrial complex I from an as-prepared sample solubilised in LMNG and reconstituted in to proteoliposomes. Frozen on Qunatifoil gold grids. Gain-corrected mages (MRC) were collected with a 200 keV FEI Talos Arctica on a Falcon 3EC detector. The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 1.69 s exposures were captured in 33 frames with a total dose of 50.37 e-/Å-2. A pixel size of 1.37 Å/pixel was used.
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