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EMPIAR-12479
Single particle cryo-EM dataset of soft-landed and rehydrated beta-galactosidase [multiple data sets in EER format]
Publication:

Cryo-EM Sample Preparation Using Soft-landing Electrospray Ion Beam Deposition and Laser Flash Melting

Barrass SV, Esser TK, Mowry NJ, Eriksson L, Hruby J, Seeley LT, Drabbels M, Baker LA, Rauschenbach S, Lorenz UJ

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Related EMDB entry:
EMD-52244 EMD-52260
Deposited:
2024-12-04
Released:
2025-12-22
Last modified:
2025-12-22
Imageset size:
24.12 TB
Imageset DOI:
10.6019/EMPIAR-12479
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
Image Sets
1. Unaligned multi-frame micrographs of soft-landed and rehydrated beta-galactosidase - grid 1
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
22957
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.726, 0.726)
Details:
EER files from a Falcon 4i detector - raw micrographs, not motion corrected
Files:
Loading...
2. Unaligned multi-frame micrographs of soft-landed and rehydrated beta-galactosidase - grid 2
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
21971
Image size:
(4096, 4096)
Pixel type:
BIT
Pixel spacing:
(0.726, 0.726)
Details:
EER files from a Falcon 4i detector - raw micrographs, not motion corrected
Files:
Loading...
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Citations
AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.
Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)
A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.
Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)
Helical reconstruction of amyloids in cryoSPARC.
Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)
An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.
Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)
CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.
Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)
prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.
He L, Bartesaghi A. (2026)
CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.
Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)
Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.
Kong L, Zottig X, Elferich J, Grigorieff N. (2026)
CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.
Gyawali R, Dhakal A, Wang L, Cheng J. (2026)
CryoWriter: a robotic solution for improved Cryo-EM grid preparation.
Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M. (2026)
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