EMPIAR-12479 - Single particle cryo-EM dataset of soft-landed and rehydrated beta-galactosidase [multiple data sets in EER format]

EMPIAR-12479

Single particle cryo-EM dataset of soft-landed and rehydrated beta-galactosidase [multiple data sets in EER format]

Publication:

Cryo-EM Sample Preparation Using Soft-landing Electrospray Ion Beam Deposition and Laser Flash Melting

Barrass SV, Esser TK, Mowry NJ, Eriksson L, Hruby J, Seeley LT, Drabbels M, Baker LA, Rauschenbach S, Lorenz UJ

(---)

Related EMDB entry:

EMD-52244 EMD-52260

Deposited:

2024-12-04

Released:

2025-12-22

Last modified:

2025-12-22

Imageset size:

24.12 TB

Imageset DOI:

10.6019/EMPIAR-12479

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of soft-landed and rehydrated beta-galactosidase - grid 1

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

22957

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

EER files from a Falcon 4i detector - raw micrographs, not motion corrected

Files:

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2. Unaligned multi-frame micrographs of soft-landed and rehydrated beta-galactosidase - grid 2

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

21971

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.726, 0.726)

Details:

EER files from a Falcon 4i detector - raw micrographs, not motion corrected

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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