EMPIAR-12146 - Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-12146

Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system

Feng Z, Yashiro Y, Tomita K

Nucleic acids research (2024)

PMID: 39228374

DOI: 10.1093/nar/gkae735

Related EMDB entry:

EMD-60564 EMD-60561 EMD-60563 EMD-60562

Deposited:

2024-06-20

Released:

2024-09-26

Last modified:

2024-09-26

Imageset size:

1.54 TB

Imageset DOI:

10.6019/EMPIAR-12146

Experimental metadata:

Download xml json

Deposited via PDBj

Contains:

micrographs - multiframe

Image Sets

1. Imageset

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

7044

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (Å/pix): 0.83
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (nm): -800 to -1800
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 50
- Please use gain.mrc as a gain reference.
- In CryoSPARC, select "flip gain ref in Y".

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Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

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Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

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He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

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Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-17]

324件のPDBエントリーが新たに公開されました。（2026-06-17付）