EMPIAR-12146
Cryo-EM structure of uropathogenic Escherichia coli CysK:CdiA:tRNA complex [7044 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:

Mechanism of activation of contact-dependent growth inhibition tRNase toxin by the amino acid biogenesis factor CysK in the bacterial competition system

Feng Z, Yashiro Y, Tomita K

Nucleic acids research (2024)

PMID: 39228374

Related EMDB entry:
Deposited:
2024-06-20
Released:
2024-09-26
Last modified:
2024-09-26
Imageset size:
1.54 TB
Imageset DOI:
Experimental metadata:
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Deposited via PDBj
Contains:
  • micrographs - multiframe
1. Imageset
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7044
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.83, 0.83)
Details:
- Microscope: FEI TITAN Krios
- Acceleration voltage (kV): 300
- Pixel size (Å/pix): 0.83
- Spherical aberration (Cs) : 2.7
- Nominal Defocus (nm): -800 to -1800
- Detector: Gatan K3 BIOQUANTUM
- Average electron dose per image (e-/A2): 50
- Number of frames per image: 50
- Please use gain.mrc as a gain reference.
- In CryoSPARC, select "flip gain ref in Y".
Files:
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Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)