EMPIAR-12124 - Cryogenic electron microscopy of chameleon-plunged acetyl-CoA decarbonylase/synthase [13892 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-12124

Cryogenic electron microscopy of chameleon-plunged acetyl-CoA decarbonylase/synthase [13892 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy

Biester A, Grahame DA, Drennan CL

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 39361653

DOI: 10.1073/pnas.2410995121

Related EMDB entry:

EMD-45090 EMD-45092 EMD-45091

Deposited:

2024-05-31

Released:

2025-02-03

Last modified:

2025-02-03

Imageset size:

1.83 TB

Imageset DOI:

10.6019/EMPIAR-12124

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

13892

Image size:

(5760, 4092)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.654, 0.654)

Details:

Gain reference:
data/GainsBackup2022-12-13/K3-18030019GainRef.x1.m1.bin2.mrc
Defocus range: 0.0 to -1.75 µM
Total exposure on specimen: 47.1 el/A2
Exposure time: 1.09 s
Calibrated pixel size: 0.654 (0.327 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3

Picked particles coordinates include:
run_data_initial_selection.star : An initial selection including a mixture of carbon monoxide dehydrogenase (CODH) alone and CODH in complex with acetyl-CoA synthase (ACS)
run_data_tetramer_selection.star : A final selection of tetramer CODH alone
run_data_pentamer_selection.star : A final selection of tetramer CODH with one ACS bound
run_data_hexamer_selection.star : A final selection of tetramer CODH with two ACSs bound

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Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）