EMPIAR-12124
Cryogenic electron microscopy of chameleon-plunged acetyl-CoA decarbonylase/synthase [13892 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Capturing a methanogenic carbon monoxide dehydrogenase/acetyl-CoA synthase complex via cryogenic electron microscopy

Biester A, Grahame DA, Drennan CL

Proceedings of the National Academy of Sciences of the United States of America 121 (2024)

PMID: 39361653

Related EMDB entry:
Deposited:
2024-05-31
Released:
2025-02-03
Last modified:
2025-02-03
Imageset size:
1.83 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of acetyl-CoA decarbonylase/synthase (ACDS) complex and subcomplexes
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
13892
Image size:
(5760, 4092)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.654, 0.654)
Details:
Gain reference:
data/GainsBackup2022-12-13/K3-18030019GainRef.x1.m1.bin2.mrc
Defocus range: 0.0 to -1.75 µM
Total exposure on specimen: 47.1 el/A2
Exposure time: 1.09 s
Calibrated pixel size: 0.654 (0.327 A super res)
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3550 V
Microscope / Detector: Krios G3i / K3

Picked particles coordinates include:
run_data_initial_selection.star : An initial selection including a mixture of carbon monoxide dehydrogenase (CODH) alone and CODH in complex with acetyl-CoA synthase (ACS)
run_data_tetramer_selection.star : A final selection of tetramer CODH alone
run_data_pentamer_selection.star : A final selection of tetramer CODH with one ACS bound
run_data_hexamer_selection.star : A final selection of tetramer CODH with two ACSs bound
Files:
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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)