EMPIAR-11662 - Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I [2540 multi-frame micrographs composed of 25 frames each in MRC format]

EMPIAR-11662

Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I [2540 multi-frame micrographs composed of 25 frames each in MRC format]

Publication:

Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation

Grba DN, Hirst J

Nature structural & molecular biology 27 (2020) 892-900

PMID: 32747785

DOI: 10.1038/s41594-020-0473-x

Related EMDB entry:

EMD-10815 EMD-10816

Deposited:

2023-08-02

Released:

2024-06-27

Last modified:

2024-06-27

Imageset size:

377.87 GB

Imageset DOI:

10.6019/EMPIAR-11662

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2540

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.055, 1.055)

Details:

Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.

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Citations

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