EMPIAR-11662
Single particle cryo-EM dataset of Yarrowia lipolytica mitochondrial complex I [2540 multi-frame micrographs composed of 25 frames each in MRC format]
Publication:

Mitochondrial complex I structure reveals ordered water molecules for catalysis and proton translocation

Grba DN, Hirst J

Nature structural & molecular biology 27 (2020) 892-900

PMID: 32747785

Related EMDB entry:
Deposited:
2023-08-02
Released:
2024-06-27
Last modified:
2024-06-27
Imageset size:
377.87 GB
Imageset DOI:
Experimental metadata:
Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (FoilHole*.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Compression was carried out by separating (“un-normalizing”) the gain.
The details of the method are described in
https://relion.readthedocs.io/en/release-3.1/Reference/MovieCompression.html#gain-normalised-movies.

Please apply the gain reference (gain-reference.mrc) during processing.

Original size:   3,531,937,314 kilobytes
Compressed size:   396,224,892 kilobytes
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM.
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
2540
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.055, 1.055)
Details:
Unaligned multi-frame micrographs of wild-type Yarrowia lipolytica mitochondrial complex I from an as-prepared sample solubilised in DDM. Frozen gold grids treated with PEGylated thiol reagent. Images (MRC) were collected with a 300 keV FEI Titan Krios on a Gatan K2 Summit detector, using counting mode and a Gatan BioContinuum energy filter (20 eV). The C2 and objective lens apertures were set to 50 and 100 µm, respectively. 10 s exposures were captured in 25 frames with a total dose of ~44 e/Å-2. A pixel size of 1.055 Å/pixel was used.
Files:
Loading...
Files:
Loading...

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)
Rigden DJ, Fernández XM. (2023)
Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)
Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)
Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)