micrographs - multiframe

TIFF

16551

(5760, 4092)

UNSIGNED BYTE

(0.663, 0.663)

This entry contains 16551 K3 movies in compressed tiff format

Collection info:
Titan Krios G3i
Voltage: 300 keV
Cs: 2.7 mm

Gatan K3 detector
130000x magnification
0.663 A/pix
20 eV energy filter
100 uM objective aperture

42.2 e/A2 over 40 frames

Gain reference: K3-GainRef-bin2.mrc
For RELION, use gain rotation 0 (no rotation) and gain flip 1 (flip upside down)


refined_particles_data.star is the run_data.star file for the final refinement

Note: many of the particles did not align well, so the final refinement used a very small fraction of the full data set. You might have better luck though!

Sample: Bombyx mori R2 retrotransposon initiating target-primed reverse transcription
Grid: Quantifoil Cu 300 mesh R2/2 + 2 nm carbon
Cemera: Gatan K3 post a BioQuantum energy filter
Camera mode: super resolution, 2-fold binning by EPU
TEM mode: EFTEM NanoProbe
Mag: x130k
C2 aperture: 50 um
Spot: 4
Beam diameter: 0.75 um
EF slit: 20 eV
Data collection software: EPU
Acquisition method: AFIS
Acquisition pattern: 9 images per hole, 12 um distance per stage shift
Physical Pixel: 0.663 A/pix
SuperRes pixel: 0.3315 A/pix
Exposure rate: 26.9 e/pix/s
Exposure time: 0.69 s
Total exposure: 42.2 e/A2
Frames: 40
Defocus: 1.5 - 2.5 um
Movies: 16551
Data format: non-gain-normalized LZW compressed TIFFs
Gain reference: "K3-GainRef-bin2.mrc"
Final particles: 39616
Particles file: "refined_particles_data.star"

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