EMPIAR-11372
Ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA [multiple data sets in TIFF format]
Publication:

Structural and biochemical basis of interdependent FANCI-FANCD2 ubiquitination

Lemonidis K, Rennie ML, Arkinson C, Chaugule VK, Clarke M, Streetley J, Walden H

The EMBO journal (2022)

PMID: 36385258

Related EMDB entry:
Deposited:
2023-01-09
Released:
2023-01-25
Last modified:
2023-01-25
Imageset size:
2.89 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5414
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.023, 1.023)
Details:
Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 45.2 e/Å^2). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in both Y and X axes.
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2. Unaligned multi-frame movies of ubiquitinated FANCI in complex with FANCD2 and double-stranded DNA (energy filtered imaging)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
5814
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.023, 1.023)
Details:
Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector and an in-column omega filter (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 46.8 e/Å^2, slit width: 30 eV). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in Y axis.
Files:
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Files:
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