Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 45.2 e/Å^2). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in both Y and X axes.
Imaging was performed on JEOL CRYO ARM 300 microscope, equipped with a DE64 detector and an in-column omega filter (Magnification: 120,000x, acceleration voltage: 300 kV, spherical aberration: 2.7 mm, electron exposure: 46.8 e/Å^2, slit width: 30 eV). Movies were recorded in MRC format and were subsequently LZW compressed in TIFF format using RELION. For CryoSPARC processing, the corresponding gain reference file needs to be flipped in Y axis.