Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 31,995 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 14,111 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 37,112 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 9,990 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.
