EMPIAR-11313
Principles of mitoribosomal small subunit assembly in eukaryotes [multiple data sets in TIFF format]
Publication:

Principles of mitoribosomal small subunit assembly in eukaryotes

Harper NJ, Burnside C, Klinge S

Nature 614 (2022) 175-181

PMID: 36482135

Deposited:
2022-10-21
Released:
2023-02-17
Last modified:
2023-02-17
Imageset size:
73.61 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of Ccm1-bound yeast mtSSU assembly intermediates
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
31995
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 31,995 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.
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2. Unaligned multi-frame micrographs of Rsm22-bound yeast mtSSU assembly intermediates
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
14111
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect 14,111 micrographs with a defocus range of -1 to -2.5 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 40 frames using a total dose of 36 e-/pixel/second (specimen pixel size of 1.08 Å/px) with an exposure time of 2 seconds and a total dose of 61.73 e-/Å^2. Gain correction was applied on the fly during collection.
Files:
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3. Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 1)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
37112
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 37,112 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.
Files:
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4. Unaligned multi-frame micrographs of METTL17-bound human mtSSU assembly intermediates (dataset 2)
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
9990
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.54, 0.54)
Details:
Grids were imaged on a Titan Krios electron microscope (FEI) with an energy filter (slit width 20 eV) and a K3 Summit detector (Gatan) operating at 300 kV with a nominal magnification of 64,000x. SerialEM was used to collect a dataset totaling 9,990 micrographs with a defocus range of -0.7 to -1.8 µm and a super-resolution pixel size of 0.54 Å. Micrographs contained 42 frames using a total dose of 28.5 e-/pixel/second with an exposure time of 2.1 seconds, resulting in a total dose of 51 e-/Å^2. Gain correction was applied on the fly during collection.
Files:
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Files:
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