EMPIAR-11221 - CryoET tilt series of mouse sperm flagella after FIB-SEM milling [69 tilt series in MRC format]

EMPIAR-11221

CryoET tilt series of mouse sperm flagella after FIB-SEM milling [69 tilt series in MRC format]

Publication:

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

Chen Z, Greenan GA, Shiozaki M, Liu Y, Skinner WM, Zhao X, Zhao S, Yan R, Yu Z, Lishko PV, Agard DA, Vale RD

Nature structural & molecular biology (2023)

PMID: 36593309

DOI: 10.1038/s41594-022-00861-0

Related EMDB entry:

EMD-27444

Deposited:

2022-09-19

Released:

2023-02-01

Last modified:

2023-02-01

Imageset size:

194.00 GB

Imageset DOI:

10.6019/EMPIAR-11221

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt series and tilt angle files

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.53, 3.53)

Details:

There are 69 tilt series with 69 tilt angle files. The number of tilt images varied because some high tilt images are blocked by ice or lost due to tracking issues.

Files:

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Citations

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Burton Smith RN, Murata K. (2026)

Structural heterogeneity of CTE Type I tau filaments with an interface-shifted polymorph

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Sun Y, Zhao J, Xu N, Wang L, Ding W, Li M. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)