EMPIAR-11221 - CryoET tilt series of mouse sperm flagella after FIB-SEM milling [69 tilt series in MRC format]

EMPIAR-11221

CryoET tilt series of mouse sperm flagella after FIB-SEM milling [69 tilt series in MRC format]

Publication:

In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes

Chen Z, Greenan GA, Shiozaki M, Liu Y, Skinner WM, Zhao X, Zhao S, Yan R, Yu Z, Lishko PV, Agard DA, Vale RD

Nature structural & molecular biology (2023)

PMID: 36593309

DOI: 10.1038/s41594-022-00861-0

Related EMDB entry:

EMD-27444

Deposited:

2022-09-19

Released:

2023-02-01

Last modified:

2023-02-01

Imageset size:

194.00 GB

Imageset DOI:

10.6019/EMPIAR-11221

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Tilt series and tilt angle files

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(4092, 5760)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.53, 3.53)

Details:

There are 69 tilt series with 69 tilt angle files. The number of tilt images varied because some high tilt images are blocked by ice or lost due to tracking issues.

Files:

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

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