EMPIAR-10908 - FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction [747 micrographs in TIFF format]

EMPIAR-10908

FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction [747 micrographs in TIFF format]

Publication:

White matter integrity requires continuous myelin synthesis at the inner tongue

Meschkat M, Steyer AM, Weil M, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave K, Möbius W

bioRxiv (2020)

DOI: 10.1101/2020.09.02.279612

Related EMDB entry:

Deposited:

2021-12-15

Released:

2022-01-12

Last modified:

2022-03-28

Imageset size:

21.53 GB

Imageset DOI:

10.6019/EMPIAR-10908

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. FIB-SEM data stack of mouse optic nerve of a conditional and inducible myelin basic protein (Mbp) knock-out 26 weeks after induction

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

747

Image size:

(7008, 4416)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(50.0, 50.0)

Details:

Mouse: 26 weeks after tamoxifen-induction, genotype: Mbpfl/fl*PlpCreERT2+, induction at the age of 8 weeks
Sample preparation: chemical fixation (2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl), rOTO embedding, Durcupan
FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3nA), z-step 50 nm
Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

ダウンロードするファイル数やサイズによっては、膨大な時間がかかります。また、この機能は不安定で正常に動作しない可能性があり、ダウンロードされたファイルのチェックサムは検証されません。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Dual site targeting of the bacterial 70S ribosome by tetracyclines.

Devarkar SC, Lomakin IB, Wang J, Grada A, Bunick CG. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

See all citations

Protein Data Bank Japan News

[2026-06-10]

333件のPDBエントリーが新たに公開されました。（2026-06-10付）