FIB-SEM of mouse optic nerve of an inducible conditional Mbp knock-out 26 weeks after induction [747 micrographs in TIFF format]
Publication:
White matter integrity requires continuous myelin synthesis at the inner tongue
Meschkat M, Steyer AM, Weil M, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Ngoc Phan NT, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave K, Möbius W
Mouse: 26 weeks after tamoxifen-induction, genotype: Mbpfl/fl*PlpCreERT2+, induction at the age of 8 weeks Sample preparation: chemical fixation (2.5 % glutaraldehyde, 4 % formaldehyde, 0.5% NaCl), rOTO embedding, Durcupan FIB-SEM: SEM: 1.5 kV, ESB grid 450V, pixel size x/y 5 nm, continuous mill-and-acquire mode; FIB: 3nA), z-step 50 nm Postprocessing: alignment with Fiji/ImageJ TrackEM2, cropped, inverted and blurred (Gaussian blur, sigma 2) with Fiji/ImageJ
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Archives and downloads the selected files into an uncompressed zip file.
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