EMPIAR-10882
Cryo electron micrographs of Tetrahymena thermophila solubilized mitochondrial membrane complexes - Glacios data [multiple data sets in TIFF and MRC formats]
Publication:

Structures of Tetrahymena's respiratory chain reveal the diversity of eukaryotic core metabolism

Zhou L, Maldonado M, Padavannil A, Guo F, Letts JA

Science (New York, N.Y.) 376 (2022) 831-839

PMID: 35357889

Related EMDB entry:
Deposited:
2021-11-22
Released:
2022-06-17
Last modified:
2022-06-17
Imageset size:
9.88 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multiframe micrographs of Tetrahymena respiratory chain complexes
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10728
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.44, 0.44)
Details:
These images correspond to the unprocessed multi-frame micrographs of three data collections on independent biological and protein isolation replicates of partially purified Tetrahymena mitochondrial respiratory chain complexes. The data were collected on a 200 kV TFS Glacios microscope using a Gatan K3 detector in super-resolution mode. The gain references are also included: SuperRef_lz_tt_bottom_001_Sep18.dm4/mrc corresponds to the data collected on September18-19 (Sep18/19 in image name) and gain.mrc corresponds to the data collected on October02-04 (Oct02/03/04 in image name).
Files:
Loading...
2. Aligned motion corrected micrographs of Tetrahymena respiratory chain complexes
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
10728
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.88, 0.88)
Details:
These are the motion corrected dose weighted and non-dose weighted micrographs. The unprocessed micrograph stacks were gain corrected, downsamples 2-times and motion corrected using MotionCorr2.
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)