EMPIAR-10882
Cryo electron micrographs of Tetrahymena thermophila solubilized mitochondrial membrane complexes - Glacios data [multiple data sets in TIFF and MRC formats]
Publication:

Structures of Tetrahymena's respiratory chain reveal the diversity of eukaryotic core metabolism

Zhou L, Maldonado M, Padavannil A, Guo F, Letts JA

Science (New York, N.Y.) 376 (2022) 831-839

PMID: 35357889

Related EMDB entry:
Deposited:
2021-11-22
Released:
2022-06-17
Last modified:
2022-06-17
Imageset size:
9.88 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
  • micrographs - single frame
1. Unaligned multiframe micrographs of Tetrahymena respiratory chain complexes
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
10728
Image size:
(11520, 8184)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.44, 0.44)
Details:
These images correspond to the unprocessed multi-frame micrographs of three data collections on independent biological and protein isolation replicates of partially purified Tetrahymena mitochondrial respiratory chain complexes. The data were collected on a 200 kV TFS Glacios microscope using a Gatan K3 detector in super-resolution mode. The gain references are also included: SuperRef_lz_tt_bottom_001_Sep18.dm4/mrc corresponds to the data collected on September18-19 (Sep18/19 in image name) and gain.mrc corresponds to the data collected on October02-04 (Oct02/03/04 in image name).
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2. Aligned motion corrected micrographs of Tetrahymena respiratory chain complexes
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
10728
Image size:
(5760, 4092)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(0.88, 0.88)
Details:
These are the motion corrected dose weighted and non-dose weighted micrographs. The unprocessed micrograph stacks were gain corrected, downsamples 2-times and motion corrected using MotionCorr2.
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