In situ cryo-electron tomography of P. chlororaphis infected by 201phi2-1 [269 multi-frame micrographs composed of 12 frames each in TIFF format]
Publication:
Architecture and self-assembly of the jumbo bacteriophage nuclear shell
Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E
Gain reference present as MRC and requires a 270 degree CW rotation prior to application to TIFs. Fluence per tilt-movie is 2.6 electrons per square Angstrom. SerialEM mdoc files stored in data/mdocs, Warp/M alignment metadata stored in data/xmls_and_tomostars, and refined ribosomes positions in data/ribosome_coordinates (as M-format .star file).