EMPIAR-10859
In situ cryo-electron tomography of P. chlororaphis infected by 201phi2-1 [269 multi-frame micrographs composed of 12 frames each in TIFF format]
Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

Related EMDB entry:
Deposited:
2021-11-06
Released:
2022-07-26
Last modified:
2022-07-26
Imageset size:
14.50 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned tilt-movie frames as unormalized LZW-TIFF
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
269
Image size:
(3710, 3838)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(3.457, 3.457)
Details:
Gain reference present as MRC and requires a 270 degree CW rotation prior to application to TIFs. Fluence per tilt-movie is 2.6 electrons per square Angstrom. SerialEM mdoc files stored in data/mdocs, Warp/M alignment metadata stored in data/xmls_and_tomostars, and refined ribosomes positions in data/ribosome_coordinates (as M-format .star file).
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