Particles were selected by 2D template matching with high-resolution templates of either M. pneumoniae or B. subtilis 50S subunits, and using a threshold with an expected false positive rate of 1 per image.
Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a M. pneumoniae 50S template
Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a B. subtilis 50S template