EMPIAR-10727
Locating Macromolecular Assemblies in Cells by 2D Template Matching with cisTEM [multiple data sets in MRC format]
Publication:

Locating macromolecular assemblies in cells by 2D template matching with cisTEM

Lucas BA, Himes BA, Xue L, Grant T, Mahamid J, Grigorieff N

eLife 10 (2021)

PMID: 34114559

Related EMDB entry:
Deposited:
2021-04-04
Released:
2021-07-07
Last modified:
2021-07-21
Imageset size:
10.99 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - single frame - unprocessed
  • picked particles - single frame - processed
1. Exposure-weighted micrographs of M. pneumoniae
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
220
Image size:
(4032, 2864)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.5, 1.5)
Details:
Particles were selected by 2D template matching with high-resolution templates of either M. pneumoniae or B. subtilis 50S subunits, and using a threshold with an expected false positive rate of 1 per image.
Files:
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2. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the M. pneumoniae 50S template
Category:
picked particles - single frame - unprocessed
Image format:
MRC
No. of images or tilt series:
5080
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.5, 1.5)
Details:
Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a M. pneumoniae 50S template
Files:
Loading...
3. Particle stack and cisTEM star file for 50S ribosomal subunits identified in the associated 220 images using the B. subtilis 50S template
Category:
picked particles - single frame - processed
Image format:
MRC
No. of images or tilt series:
1172
Image size:
(256, 256)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.5, 1.5)
Details:
Particle stack and cisTEM star file of 50S ribosomal subunits identified in the associated 220 images of M. pneumoniae, using a B. subtilis 50S template
Files:
Loading...
Files:
Loading...

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