EMPIAR-10695 - Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan [multiple data sets in TIFF and MRC formats]

EMPIAR-10695

Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan [multiple data sets in TIFF and MRC formats]

Publication:

Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling

van der Stel AX, Gordon ER, Sengupta A, Martínez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vázquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nature communications 12 (2021)

PMID: 34504068

DOI: 10.1038/s41467-021-25663-8

Related EMDB entry:

EMD-12693 EMD-12694 EMD-12695

Deposited:

2021-03-29

Released:

2022-01-24

Last modified:

2022-01-24

Imageset size:

3.75 TB

Imageset DOI:

10.6019/EMPIAR-10695

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Raw multiframe micrographs of TnaC-WT-70S ribosome stalled on L-Trp

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8964

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.907, 0.907)

Details:

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixelsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-WT-70S sample as described in the original reference. These micrographs were used to reconstruct the map EMDB-12693 and corresponding molecular model PDB-7O19. This map and model contain the 70S ribosome stalled on TnaC in response to L-tryptophan All image processing has been performed using RELION3.1

Files:

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2. Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset B)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8743

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.907, 0.907)

Details:

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-(R23F)-70S sample as described in the original reference.
This dataset (TnaC-(R23F)-70S_B) was used together with the dataset (TnaC-(R23F)-70S_A) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation.
All image processing has been performed using RELION3.1

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset A)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7867

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.835, 0.835)

Details:

Raw micrographs collected on a Titan KRIOS (ESRF-Grenoble) with a nominal pixsize of 0.827A/px (calibrated 0.835A/px) of the TnaC-(R23F)-70S sample as described in the original reference.
This dataset (TnaC-(R23F)-70S_B) was used to reconstruct the map EMDB-12694 and corresponding molecular model PDB-7O1A. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan.
In addition, this dataset was also used together with the dataset (TnaC-(R23F)-70S_B) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation.
All image processing has been performed using RELION3.1

Files:

選択したファイルを無圧縮zipファイルにアーカイブ化してダウンロードします。

rsync、aspera、globus 等の利用をおすすめします。

選択したファイルのリストをダウンロードします。

rsyncコマンド等でファイルリストを指定してダウンロードすることが可能です。

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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