EMPIAR-10695 - Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan [multiple data sets in TIFF and MRC formats]

EMPIAR-10695

Cryo-EM structure of an Escherichia coli TnaC-ribosome complex stalled in response to L-tryptophan [multiple data sets in TIFF and MRC formats]

Publication:

Structural basis for the tryptophan sensitivity of TnaC-mediated ribosome stalling

van der Stel AX, Gordon ER, Sengupta A, Martínez AK, Klepacki D, Perry TN, Herrero Del Valle A, Vázquez-Laslop N, Sachs MS, Cruz-Vera LR, Innis CA

Nature communications 12 (2021)

PMID: 34504068

DOI: 10.1038/s41467-021-25663-8

Related EMDB entry:

EMD-12693 EMD-12694 EMD-12695

Deposited:

2021-03-29

Released:

2022-01-24

Last modified:

2022-01-24

Imageset size:

3.75 TB

Imageset DOI:

10.6019/EMPIAR-10695

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (data/TnaC_R23F_KRIOS_ESRF_raw_micrographs/FoilHole*.mrc) in this entry were compressed to TIFF by
EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   4,375,715,871 kilobytes
Compressed size:   940,306,259 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Raw multiframe micrographs of TnaC-WT-70S ribosome stalled on L-Trp

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8964

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.907, 0.907)

Details:

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixelsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-WT-70S sample as described in the original reference. These micrographs were used to reconstruct the map EMDB-12693 and corresponding molecular model PDB-7O19. This map and model contain the 70S ribosome stalled on TnaC in response to L-tryptophan All image processing has been performed using RELION3.1

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2. Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset B)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8743

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.907, 0.907)

Details:

Raw micrographs collected on a Talos Arctica (IECB-Bordeaux) with a nominal pixsize of 0.93A/px (calibrated 0.907A/px) of the TnaC-(R23F)-70S sample as described in the original reference.
This dataset (TnaC-(R23F)-70S_B) was used together with the dataset (TnaC-(R23F)-70S_A) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation.
All image processing has been performed using RELION3.1

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Raw multiframe micrographs of TnaC-R23F-70S ribosome stalled on L-Trp (dataset A)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

7867

Image size:

(3838, 3710)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.835, 0.835)

Details:

Raw micrographs collected on a Titan KRIOS (ESRF-Grenoble) with a nominal pixsize of 0.827A/px (calibrated 0.835A/px) of the TnaC-(R23F)-70S sample as described in the original reference.
This dataset (TnaC-(R23F)-70S_B) was used to reconstruct the map EMDB-12694 and corresponding molecular model PDB-7O1A. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan.
In addition, this dataset was also used together with the dataset (TnaC-(R23F)-70S_B) to reconstruct the map EMDB-12695 and corresponding molecular model PDB-7O1C. This map and model contains the 70S ribosome stalled on TnaC-(R23F) in response to L-tryptophan in complex with release factor 2 (RF2) in the A-site in a near-active conformation.
All image processing has been performed using RELION3.1

Files:

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