EMPIAR-10392
Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis [multiple data sets in MRC and TIFF formats]
Publication:

Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis

Rudlaff RM, Kraemer S, Marshman J, Dvorin JD

PLoS pathogens 16 (2020)

PMID: 32511279

Related EMDB entry:
Deposited:
2020-05-13
Released:
2020-05-14
Last modified:
2021-02-12
Imageset size:
3.94 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. S1_File_Early_Segmentation_1
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
311
Image size:
(530, 810)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
File S1: Volume EM of Plasmodium falciparum 3D7 early schizont 1. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
2. S2_File_Early_Segmentation_2
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
397
Image size:
(773, 607)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S2: Volume EM of Plasmodium falciparum 3D7 early schizont 2. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
3. S3_File_Early_Segmentation_A
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
427
Image size:
(611, 805)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S3: Volume EM of Plasmodium falciparum 3D7 early schizont A. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
4. S4_File_Early_Segmentation_B
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
382
Image size:
(621, 798)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S4: Volume EM of Plasmodium falciparum 3D7 early schizont B. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
5. S5_File_Mid_Segmentation_1
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
278
Image size:
(655, 718)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
File S5: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 1. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
6. S6_File_Mid_Segmentation_2
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
278
Image size:
(657, 726)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S6: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 2. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
7. S7_File_Mid_Segmentation_A
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
249
Image size:
(838, 701)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S7: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont A. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
8. S8_File_Mid_Segmentation_B
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
290
Image size:
(830, 651)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(100.0, 100.0)
Details:
File S8: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont B. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
9. S9_File_PVM_Rupture
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
190
Image size:
(617, 617)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
File S9: Volume EM of Plasmodium falciparum 3D7 schizont at PVM rupture. Aligned, denoised, binned, and cropped serial slices of a schizont at the PVM rupture stage from the [+]E64 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).
Files:
Loading...
10. S10_File_Post_PVM_Rupture_E64
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
306
Image size:
(684, 649)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(100.0, 100.0)
Details:
File S10: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [+]E64-treated block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. cellular debris).
Files:
Loading...
11. S11_File_Post_PVM_Rupture_NoE64
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
339
Image size:
(1005, 870)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(100.0, 100.0)
Details:
File S11: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [-]E64-treated block. Voxels are 10nm x 10nm x 20nm. The image quality of this cell is relatively lower; thus, it was not rendered.
Files:
Loading...
Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)
Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)
Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)
Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)