EMPIAR-10392 - Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis [multiple data sets in MRC and TIFF formats]

EMPIAR-10392

Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis [multiple data sets in MRC and TIFF formats]

Publication:

Three-dimensional ultrastructure of Plasmodium falciparum throughout cytokinesis

Rudlaff RM, Kraemer S, Marshman J, Dvorin JD

PLoS pathogens 16 (2020)

PMID: 32511279

DOI: 10.1371/journal.ppat.1008587

Related EMDB entry:

Deposited:

2020-05-13

Released:

2020-05-14

Last modified:

2021-02-12

Imageset size:

3.94 GB

Imageset DOI:

10.6019/EMPIAR-10392

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. S1_File_Early_Segmentation_1

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

311

Image size:

(530, 810)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

File S1: Volume EM of Plasmodium falciparum 3D7 early schizont 1. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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2. S2_File_Early_Segmentation_2

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

397

Image size:

(773, 607)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S2: Volume EM of Plasmodium falciparum 3D7 early schizont 2. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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3. S3_File_Early_Segmentation_A

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

427

Image size:

(611, 805)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S3: Volume EM of Plasmodium falciparum 3D7 early schizont A. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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4. S4_File_Early_Segmentation_B

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

382

Image size:

(621, 798)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S4: Volume EM of Plasmodium falciparum 3D7 early schizont B. Aligned, denoised, binned, and cropped serial slices of an early schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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5. S5_File_Mid_Segmentation_1

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

278

Image size:

(655, 718)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

File S5: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 1. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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6. S6_File_Mid_Segmentation_2

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

278

Image size:

(657, 726)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S6: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont 2. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [+]E64-treated 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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7. S7_File_Mid_Segmentation_A

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

249

Image size:

(838, 701)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S7: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont A. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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8. S8_File_Mid_Segmentation_B

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

290

Image size:

(830, 651)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(100.0, 100.0)

Details:

File S8: Volume EM of Plasmodium falciparum 3D7 mid-segmentation schizont B. Aligned, denoised, binned, and cropped serial slices of a mid-segmentation schizont from the [-]E64 46-48hpi block. Voxels 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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9. S9_File_PVM_Rupture

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

190

Image size:

(617, 617)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

File S9: Volume EM of Plasmodium falciparum 3D7 schizont at PVM rupture. Aligned, denoised, binned, and cropped serial slices of a schizont at the PVM rupture stage from the [+]E64 48-50hpi block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. PVM).

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10. S10_File_Post_PVM_Rupture_E64

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

306

Image size:

(684, 649)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(100.0, 100.0)

Details:

File S10: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [+]E64-treated block. Voxels are 10nm x 10nm x 20nm. Additional ultrastructural features are present in the raw data that were not rendered (e.g. cellular debris).

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11. S11_File_Post_PVM_Rupture_NoE64

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

339

Image size:

(1005, 870)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(100.0, 100.0)

Details:

File S11: Volume EM of Plasmodium falciparum 3D7 post-PVM rupture schizont. Aligned, denoised, binned, and cropped serial slices of a post-PVM rupture schizont from the [-]E64-treated block. Voxels are 10nm x 10nm x 20nm. The image quality of this cell is relatively lower; thus, it was not rendered.

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