EMPIAR-10859 - In situ cryo-electron tomography of P. chlororaphis infected by 201phi2-1 [269 multi-frame micrographs composed of 12 frames each in TIFF format]

EMPIAR-10859

In situ cryo-electron tomography of P. chlororaphis infected by 201phi2-1 [269 multi-frame micrographs composed of 12 frames each in TIFF format]

Publication:

Architecture and self-assembly of the jumbo bacteriophage nuclear shell

Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E

bioRxiv (2022)

DOI: 10.1101/2022.02.14.480162

Related EMDB entry:

EMD-25220 EMD-25221 EMD-25183 EMD-25222

Deposited:

2021-11-06

Released:

2022-07-26

Last modified:

2022-07-26

Imageset size:

14.50 GB

Imageset DOI:

10.6019/EMPIAR-10859

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned tilt-movie frames as unormalized LZW-TIFF

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

269

Image size:

(3710, 3838)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(3.457, 3.457)

Details:

Gain reference present as MRC and requires a 270 degree CW rotation prior to application to TIFs. Fluence per tilt-movie is 2.6 electrons per square Angstrom. SerialEM mdoc files stored in data/mdocs, Warp/M alignment metadata stored in data/xmls_and_tomostars, and refined ribosomes positions in data/ribosome_coordinates (as M-format .star file).

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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